Author: Narendrula, Rashmi; Mispel-Beyer, Kyle; Guo, Baoqing; Parissenti, Amadeo M.; Pritzker, Laura B.; Pritzker, Ken; Masilamani, Twinkle; Wang, Xiaohui; Lannér, Carita
Title: RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines Document date: 2016_2_24
ID: 0mjizsoo_29
Snippet: The abnormal RNA disruption bands that occur upon chemotherapy drug exposure are smaller in molecular weight than the 28S and/or 18S rRNAs. To determine whether the abnormal bands originate from the 28S and/or 18S rRNAs, Northern blotting experiments were performed on total RNA prepared from A2780 cells after incubation in the absence or presence of docetaxel for up to 48 h (Fig. 4) . Of the probes complementary to the 28S rRNA, only probe 28S-5 .....
Document: The abnormal RNA disruption bands that occur upon chemotherapy drug exposure are smaller in molecular weight than the 28S and/or 18S rRNAs. To determine whether the abnormal bands originate from the 28S and/or 18S rRNAs, Northern blotting experiments were performed on total RNA prepared from A2780 cells after incubation in the absence or presence of docetaxel for up to 48 h (Fig. 4) . Of the probes complementary to the 28S rRNA, only probe 28S-5 hybridized to RNA disruption bands (Fig. 4 , Additional files 2 and 3). Figure 4a shows hybridization of the 28S-5 probe to the 28S rRNA and to two smaller bands computed to be 3012 nt and 1630 nt in length. Both of these RNA disruption bands were derived from the 3'end of the 28S rRNA sequence,
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