Selected article for: "amino acid and gene tree"
Author: Abdel-Moneim, Ahmed S; El-Kady, Magdy F; Ladman, Brian S; Gelb, Jack
Title: S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
  • Document date: 2006_9_20
    • ID: 1in7m56w_13
    Snippet: In this study, an Egyptian IBV strain; Egypt\F/03 was isolated from a tissue pool of kidney and trachea from unvaccinated broiler flock with a history of respiratory and renal disease. The strain produced typical lesions of IBV in inoculated embryos and identified as IBV by Dot-ELISA and RT-PCR. The isolate was found to be devoid of major concomitant viruses; avian influenza virus, Newcastle disease virus, infectious laryngotracheitis virus, reov.....
    Document: In this study, an Egyptian IBV strain; Egypt\F/03 was isolated from a tissue pool of kidney and trachea from unvaccinated broiler flock with a history of respiratory and renal disease. The strain produced typical lesions of IBV in inoculated embryos and identified as IBV by Dot-ELISA and RT-PCR. The isolate was found to be devoid of major concomitant viruses; avian influenza virus, Newcastle disease virus, infectious laryngotracheitis virus, reovirus and adenovirus (data not shown). S1 sequence analysis of Egypt/F/03 revealed its close relatedness to Mass serotype. It showed high nucleotide similarities to GX1-98.China (99% nucleotide and amino acid identities), Beaudette-US (98% nucleotide and 96% amino acid identities), IS/385/97 (98% nucleotide and 97% amino acid identities), H120 (97% nucleotide and amino acid identities) and M41 (98% nucleotide and 96% amino acid identities). IBV S1 gene sequence relationships expressed as a phyloge-netic tree of Egypt/F/03 isolate and selected IBV reference strains Figure 2 IBV S1 gene sequence relationships expressed as a phylogenetic tree of Egypt/F/03 isolate and selected IBV reference strains.

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