Author: Laine, Romain F.; Albecka, Anna; van de Linde, Sebastian; Rees, Eric J.; Crump, Colin M.; Kaminski, Clemens F.
Title: Structural analysis of herpes simplex virus by optical super-resolution imaging Document date: 2015_1_22
ID: 0zchxz00_18
Snippet: The reduction in the number of localizations that was observed when comparing the secondary with primary and Fab labelling strategies can be attributed to the reduction of the average number of fluorescent labels per antibody, from 3 to 4 on commercial secondary antibody to 1.0-1.5 for in-house labelled primary antibodies and Fab fragments. In addition, multiple secondary antibodies may bind to a single primary antibody. This would also contribut.....
Document: The reduction in the number of localizations that was observed when comparing the secondary with primary and Fab labelling strategies can be attributed to the reduction of the average number of fluorescent labels per antibody, from 3 to 4 on commercial secondary antibody to 1.0-1.5 for in-house labelled primary antibodies and Fab fragments. In addition, multiple secondary antibodies may bind to a single primary antibody. This would also contribute to the greater number of localizations observed with secondary labelling.
Search related documents:
Co phrase search for related documents- average number and great number: 1, 2, 3, 4
- fab fragment and primary antibody: 1
- fab fragment and secondary antibody: 1
- fab fragment and secondary labelling: 1
- fab labelling and fluorescent label: 1
- fab labelling and primary antibody: 1
- fab labelling and secondary antibody: 1
- fab labelling and secondary labelling: 1, 2
- fluorescent label and secondary labelling: 1
- multiple secondary antibody and secondary antibody: 1
- primary antibody and secondary antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- primary antibody and secondary labelling: 1
- primary antibody and single primary antibody: 1
Co phrase search for related documents, hyperlinks ordered by date