Selected article for: "allantoic fluid and th passage"
Author: Abdel-Moneim, Ahmed S; El-Kady, Magdy F; Ladman, Brian S; Gelb, Jack
Title: S1 gene sequence analysis of a nephropathogenic strain of avian infectious bronchitis virus in Egypt
  • Document date: 2006_9_20
    • ID: 1in7m56w_30
    Snippet: Egypt/F/03 was isolated from 25-day-old broiler chickens suffering from both respiratory and renal distresses from Fayoum Governorate in 2003. A kidney homogenate (10% in sterile PBS) and a tracheal scraping suspension were pooled, centrifuged at 500 × g for 10 min. The supernatant fluid was inoculated into chorioallantoic sac of 10day-old SPF ECE. Allantoic fluid was harvested after 48 h and was used for re-passage into ECE. Five eggs of the 4 .....
    Document: Egypt/F/03 was isolated from 25-day-old broiler chickens suffering from both respiratory and renal distresses from Fayoum Governorate in 2003. A kidney homogenate (10% in sterile PBS) and a tracheal scraping suspension were pooled, centrifuged at 500 × g for 10 min. The supernatant fluid was inoculated into chorioallantoic sac of 10day-old SPF ECE. Allantoic fluid was harvested after 48 h and was used for re-passage into ECE. Five eggs of the 4 th egg passage were incubated till being 18-day-old and examined for typical lesions of IBV (stunting, curling and urates deposition in ureters).

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