Selected article for: "endogenous control and real time pcr"
Author: Frieman, Matthew B.; Chen, Jun; Morrison, Thomas E.; Whitmore, Alan; Funkhouser, William; Ward, Jerrold M.; Lamirande, Elaine W.; Roberts, Anjeanette; Heise, Mark; Subbarao, Kanta; Baric, Ralph S.
Title: SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism
  • Document date: 2010_4_8
    • ID: 15rtwl26_64
    Snippet: Lungs from mock or SARS-CoV infected mice were removed and homogenized directly in 1 ml of Trizol reagent (Invitrogen) and total RNA was isolated following manufacturer's instructions. Complementary cDNA was generated from 1 mg of total RNA using 250 ng random primers (Invitrogen) and Superscript II reverse transcriptase (Invitrogen). Real-time PCR experiments were performed using Taqman gene expression assays and an ABI Prism 7300 (Applied Biosy.....
    Document: Lungs from mock or SARS-CoV infected mice were removed and homogenized directly in 1 ml of Trizol reagent (Invitrogen) and total RNA was isolated following manufacturer's instructions. Complementary cDNA was generated from 1 mg of total RNA using 250 ng random primers (Invitrogen) and Superscript II reverse transcriptase (Invitrogen). Real-time PCR experiments were performed using Taqman gene expression assays and an ABI Prism 7300 (Applied Biosystems). 18S rRNA was used as an endogenous control to use for normalization in all assays. The relative fold induction of amplified mRNA were detected using the Ct method. Taqman primer sets used were 18S (#Hs03003631

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