Selected article for: "detection limit and Vero cell"

Author: Frieman, Matthew B.; Chen, Jun; Morrison, Thomas E.; Whitmore, Alan; Funkhouser, William; Ward, Jerrold M.; Lamirande, Elaine W.; Roberts, Anjeanette; Heise, Mark; Subbarao, Kanta; Baric, Ralph S.
Title: SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism
  • Document date: 2010_4_8
  • ID: 15rtwl26_57
    Snippet: To quantify the amount of infectious virus in tissues from rMA15 infection, each organ was weighed, placed in 0.5 ml DPBS, homogenized, and titered via plaque assay on Vero E6 cells as previously described [26] . For Urbani infection, supernatants of 10% (w/v) lung homogenates were prepared and titrated on Vero cell monolayers in 24-and 96-well plates as previously described [40] . Virus titers are expressed as TCID 50 per g of tissue. The lower .....
    Document: To quantify the amount of infectious virus in tissues from rMA15 infection, each organ was weighed, placed in 0.5 ml DPBS, homogenized, and titered via plaque assay on Vero E6 cells as previously described [26] . For Urbani infection, supernatants of 10% (w/v) lung homogenates were prepared and titrated on Vero cell monolayers in 24-and 96-well plates as previously described [40] . Virus titers are expressed as TCID 50 per g of tissue. The lower limit of detection was 10 1.5 TCID 50 /g.

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