Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_33
Snippet: Single-step growth curve analysis. To analyze viral replication kinetics, a single-step growth curve was determined as previously described (53, 54) . Briefly, Vero cells in six-well plates were infected with the HSV-1 gB 3A-1, HSV-1 3A-2, or GS3217 (WT) virus at 5 PFU/cell for 1 h. The inoculum was removed, and the cells were rinsed twice with PBS and then incubated at 37°C (time zero, binding time not included). After 6, 12, 18, 24, and 48 h o.....
Document: Single-step growth curve analysis. To analyze viral replication kinetics, a single-step growth curve was determined as previously described (53, 54) . Briefly, Vero cells in six-well plates were infected with the HSV-1 gB 3A-1, HSV-1 3A-2, or GS3217 (WT) virus at 5 PFU/cell for 1 h. The inoculum was removed, and the cells were rinsed twice with PBS and then incubated at 37°C (time zero, binding time not included). After 6, 12, 18, 24, and 48 h of incubation, cells were harvested and freeze-thawed at Ϫ40°C three times and virus titers were determined by plaque assay on Vero cells.
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