Selected article for: "PCR analysis and real time"
Author: Frieman, Matthew B.; Chen, Jun; Morrison, Thomas E.; Whitmore, Alan; Funkhouser, William; Ward, Jerrold M.; Lamirande, Elaine W.; Roberts, Anjeanette; Heise, Mark; Subbarao, Kanta; Baric, Ralph S.
Title: SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism
  • Document date: 2010_4_8
    • ID: 15rtwl26_27
    Snippet: We used real time RT-PCR and CBA analysis to quantify changes in mRNA and protein expression levels of several innate immune factors involved in pulmonary inflammation. Specifically, we compared the induction of several pro-inflammatory cytokines (TNFa, IL6, IFNc and MCP1) for changes in expression during infection by analyzing lung homogenates by CBA ( Figure 4A , B, C and D) or quantitative RT-PCR to measure levels of mRNAs in Urbani virus or r.....
    Document: We used real time RT-PCR and CBA analysis to quantify changes in mRNA and protein expression levels of several innate immune factors involved in pulmonary inflammation. Specifically, we compared the induction of several pro-inflammatory cytokines (TNFa, IL6, IFNc and MCP1) for changes in expression during infection by analyzing lung homogenates by CBA ( Figure 4A , B, C and D) or quantitative RT-PCR to measure levels of mRNAs in Urbani virus or rMA15 virus infected mice, respectively (Figure 4E , F, G and H).

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