Author: Stagegaard, Julia; Kurth, Andreas; Stern, Daniel; Dabrowski, Piotr Wojciech; Pocknell, Ann; Nitsche, Andreas; Schrick, Livia
Title: Seasonal recurrence of cowpox virus outbreaks in captive cheetahs (Acinonyx jubatus) Document date: 2017_11_9
ID: 0oa4x05s_8
Snippet: Full viral genome sequences were gained from one specimen of each cluster by using either DNA from cell culture isolates (if available) or DNA directly isolated from crust or skin tissue. Samples from clusters 1-4 were subjected to IonTorrent PGM sequencing (Ion Xpress Plus Fragment Preparation Kit, Ion PGMâ„¢ Sequencing 200 Kit, Ion 318 Chip). For the sample from cluster 5 Illumina sequencing was utilized (Nextera library, TruSeq Rapid PE Cluste.....
Document: Full viral genome sequences were gained from one specimen of each cluster by using either DNA from cell culture isolates (if available) or DNA directly isolated from crust or skin tissue. Samples from clusters 1-4 were subjected to IonTorrent PGM sequencing (Ion Xpress Plus Fragment Preparation Kit, Ion PGMâ„¢ Sequencing 200 Kit, Ion 318 Chip). For the sample from cluster 5 Illumina sequencing was utilized (Nextera library, TruSeq Rapid PE Cluster Kit v2, HiSeq Rapid SBS Kit v2, 250+250 bases sequencing on a HiSeq 1500 instrument in rapid mode).
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