Author: Guo, Jiao; Jia, Xiaoying; Liu, Yang; Wang, Shaobo; Cao, Junyuan; Zhang, Bo; Xiao, Gengfu; Wang, Wei
Title: Screening of Natural Extracts for Inhibitors against Japanese Encephalitis Virus Infection Document date: 2020_2_21
ID: 161ck1i9_16
Snippet: The viral burden was determined by plaque assay. The samples were weighed and homogenized in 300 l of PBS. The homogenates were clarified by centrifugation (2,000 ϫ g at 4°C) for 15 min and then diluted serially prior to infection of BHK-21 cells. The viral titer was adjusted for sample volume and tissue weight to calculate the titer as PFU/g. Tissue samples and blood from JEV-infected mice were extracted with the RNAprep pure tissue kit (Tiang.....
Document: The viral burden was determined by plaque assay. The samples were weighed and homogenized in 300 l of PBS. The homogenates were clarified by centrifugation (2,000 ϫ g at 4°C) for 15 min and then diluted serially prior to infection of BHK-21 cells. The viral titer was adjusted for sample volume and tissue weight to calculate the titer as PFU/g. Tissue samples and blood from JEV-infected mice were extracted with the RNAprep pure tissue kit (Tiangen; catalog no. DP431) and RNAprep pure blood kit (Tiangen; catalog no. DP433), respectively. JEV RNA levels were determined by one-step qRT-PCR on ABI Real-Time PCR systems using standard cycling conditions. The viral burden was calculated on a standard curve produced using serial 10-fold dilutions of plasmids carrying the infectious clone.
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