Author: Stagegaard, Julia; Kurth, Andreas; Stern, Daniel; Dabrowski, Piotr Wojciech; Pocknell, Ann; Nitsche, Andreas; Schrick, Livia
Title: Seasonal recurrence of cowpox virus outbreaks in captive cheetahs (Acinonyx jubatus) Document date: 2017_11_9
ID: 0oa4x05s_6
Snippet: DNA was extracted from multiple specimens by using the QIAGEN QIAamp DNA Mini Kit. The DNA was analyzed by OPV-specific real-time PCR (rpo 18) [13] , CPXV-specific real-time PCR [14] , and real-time PCR detecting the cellular c-myc gene for relative quantification [15] . Additionally, conventional PCR was performed, amplifying the open reading frame (ORF) of the hemagglutinin (HA) gene followed by sanger sequencing [15] ......
Document: DNA was extracted from multiple specimens by using the QIAGEN QIAamp DNA Mini Kit. The DNA was analyzed by OPV-specific real-time PCR (rpo 18) [13] , CPXV-specific real-time PCR [14] , and real-time PCR detecting the cellular c-myc gene for relative quantification [15] . Additionally, conventional PCR was performed, amplifying the open reading frame (ORF) of the hemagglutinin (HA) gene followed by sanger sequencing [15] .
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