Selected article for: "PCR assay and quantitative PCR assay"
Author: Guo, Jiao; Jia, Xiaoying; Liu, Yang; Wang, Shaobo; Cao, Junyuan; Zhang, Bo; Xiao, Gengfu; Wang, Wei
Title: Screening of Natural Extracts for Inhibitors against Japanese Encephalitis Virus Infection
  • Document date: 2020_2_21
    • ID: 161ck1i9_5
    Snippet: Under the same conditions of infection, ouabain and digoxin robustly inhibited AT31 and SA14 virus production in a plaque assay, with a reduction of approximately 4 to 6 log units at the highest concentration ( Fig. 3C and E) . A sharp decrease in JEV RNA levels was also detected with a quantitative reverse transcription PCR (qRT-PCR) assay. In particular, the attenuated RNA levels of the SA14 strain in the high-dose-, middle-dose-, and low-dose-.....
    Document: Under the same conditions of infection, ouabain and digoxin robustly inhibited AT31 and SA14 virus production in a plaque assay, with a reduction of approximately 4 to 6 log units at the highest concentration ( Fig. 3C and E) . A sharp decrease in JEV RNA levels was also detected with a quantitative reverse transcription PCR (qRT-PCR) assay. In particular, the attenuated RNA levels of the SA14 strain in the high-dose-, middle-dose-, and low-dose-treated groups were all above 40%, indicating a strong inhibition of viral replication ( Fig. 3D and F) .

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