Author: Frieman, Matthew B.; Chen, Jun; Morrison, Thomas E.; Whitmore, Alan; Funkhouser, William; Ward, Jerrold M.; Lamirande, Elaine W.; Roberts, Anjeanette; Heise, Mark; Subbarao, Kanta; Baric, Ralph S.
Title: SARS-CoV Pathogenesis Is Regulated by a STAT1 Dependent but a Type I, II and III Interferon Receptor Independent Mechanism Document date: 2010_4_8
ID: 15rtwl26_53
Snippet: Vero E6 cells were grown in MEM (Invitrogen, Carlsbad, CA) supplemented with 10% FCII (Hyclone, South Logan, UT) and gentamicin/kanamycin (Gibco-BRL). Stocks of the biological SARS-CoV (Urbani), recombinant SARS-CoV (icSARS) and mouse-adapted SARS-CoV (rMA15) were propagated and titered on Vero or Vero E6 cells and cryopreserved at 280uC until use as described [30] . All experiments with infectious virus were performed in a Class II biological sa.....
Document: Vero E6 cells were grown in MEM (Invitrogen, Carlsbad, CA) supplemented with 10% FCII (Hyclone, South Logan, UT) and gentamicin/kanamycin (Gibco-BRL). Stocks of the biological SARS-CoV (Urbani), recombinant SARS-CoV (icSARS) and mouse-adapted SARS-CoV (rMA15) were propagated and titered on Vero or Vero E6 cells and cryopreserved at 280uC until use as described [30] . All experiments with infectious virus were performed in a Class II biological safety cabinet in a certified biosafety level 3 laboratory containing redundant exhaust fans with personnel wearing protective equipment including Tyvek suits, hoods, and HEPA-filtered powered air-purifying respirators (PAPRs) as described [30] .
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