RNA disruption is associated with response to multiple classes of chemotherapy drugs in tumor cell lines

Narendrula, Rashmi; Mispel-Beyer, Kyle; Guo, Baoqing; Parissenti, Amadeo M.; Pritzker, Laura B.; Pritzker, Ken; Masilamani, Twinkle; Wang, Xiaohui; Lannér, Carita

Carboplatin, a structurally distinct drug with a very different mechanism of action from taxanes, required a longer exposure time to induce RNA disruption in A2780 and CaOV3 cells. Therefore, A2780 and CaOV3 cells were treated for 72 h with increasing doses of carboplatin. Figure 2a and b display RNA electropherograms and RDI values of A2780 cells treated with 0.001 to 100 μM carboplatin. For A2780 cells, RNA disruption became detectable and significantly higher beginning at 5 μM carboplatin. For CaOV3 cells, RNA disruption was detectable at 10 μM carboplatin but RDI values did not become significantly higher compared to untreated cells until 50 and 100 μM carboplatin were used ( Fig. 2c and d). -ACG TGT TAG ACT CCT TGG TCC GTG-3