Author: Zhang, Chi; Xiu, Leshan; Xiao, Yan; Xie, Zhengde; Ren, Lili; Peng, Junping
Title: Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry Document date: 2018_4_5
ID: 0k2blp7n_34
Snippet: Using conventional and real-time PCR techniques, microbial etiologies can be identified in 65 to 86% of CAP cases (Thomson and Harris, 2011) . For bacterial etiologies, detection rates are reported to be 58% by Johansson et al. (2010) and 44.7% by Jones (2010) . Still nearly half of pneumonia cases, in which no causative agents can be detected (Mandell et al., 2007) . In the present study, using the BP-MS method, the rate of bacterial pathogen de.....
Document: Using conventional and real-time PCR techniques, microbial etiologies can be identified in 65 to 86% of CAP cases (Thomson and Harris, 2011) . For bacterial etiologies, detection rates are reported to be 58% by Johansson et al. (2010) and 44.7% by Jones (2010) . Still nearly half of pneumonia cases, in which no causative agents can be detected (Mandell et al., 2007) . In the present study, using the BP-MS method, the rate of bacterial pathogen detection was 81.9% (167/204), which indicates that this new method can assist determining the causative pathogens in cases with unknown etiologies.
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