Selected article for: "analytical sensitivity and clinical testing"
Author: Zhang, Chi; Xiu, Leshan; Xiao, Yan; Xie, Zhengde; Ren, Lili; Peng, Junping
Title: Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry
  • Document date: 2018_4_5
    • ID: 0k2blp7n_23
    Snippet: Specificity of the method was validated by testing 58 confirmed clinical samples and isolates ( Table 1) . The results showed that the BP-MS method can be used to accurately identify the expected bacteria without cross-reactivity with non-target pathogens (Figures S1, S2 in the Supplementary Material). Plasmids containing the target genes of each pathogen were 10-fold diluted and used to evaluate the analytical sensitivity of the BP-MS method. Ba.....
    Document: Specificity of the method was validated by testing 58 confirmed clinical samples and isolates ( Table 1) . The results showed that the BP-MS method can be used to accurately identify the expected bacteria without cross-reactivity with non-target pathogens (Figures S1, S2 in the Supplementary Material). Plasmids containing the target genes of each pathogen were 10-fold diluted and used to evaluate the analytical sensitivity of the BP-MS method. Based on the results, the BP-MS method can be used to determine the concentration of corresponding plasmids with 10 copies per reaction (Figure 1) .

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