Selected article for: "anomalous diffraction and electron density"
Author: Hao, Wei; Wojdyla, Justyna Aleksandra; Zhao, Rong; Han, Ruiyun; Das, Rajat; Zlatev, Ivan; Manoharan, Muthiah; Wang, Meitian; Cui, Sheng
Title: Crystal structure of Middle East respiratory syndrome coronavirus helicase
  • Document date: 2017_6_26
    • ID: 0vxhgjss_13
    Snippet: Our crystallization trials with the unliganded MERS-CoV nsp13 yielded crystal, which diffracted the X-rays poorly. Intriguingly, incubation with a 5'-triphosphate-15T DNA (ppp-15T) greatly improved diffracting power of nsp13 crystals. Benefiting from the presence of an N-terminal zinc-binding domain, we collected highly redundant multi-wavelength anomalous diffraction (MAD) data at the zinc absorption edge and subsequently solved the structure. N.....
    Document: Our crystallization trials with the unliganded MERS-CoV nsp13 yielded crystal, which diffracted the X-rays poorly. Intriguingly, incubation with a 5'-triphosphate-15T DNA (ppp-15T) greatly improved diffracting power of nsp13 crystals. Benefiting from the presence of an N-terminal zinc-binding domain, we collected highly redundant multi-wavelength anomalous diffraction (MAD) data at the zinc absorption edge and subsequently solved the structure. Next, we collected a 3.0Ã… resolution native dataset, which we used for final structure refinement and further analysis. There are two nsp13 in the asymmetric unit (ASU) with three zinc ions bound to each N-terminal Cys/His rich domain. Unexpectedly, no additional electron density for ppp-15T DNA could be identified, indicating that a stable nsp13-DNA complex did not form. The final model of nsp13 has good stereochemistry quality. Data collection and refinement statistics are summarized in Table 1 .

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