Author: Xu, Xiaoling; Lou, Zhiyong; Ma, Yanlin; Chen, Xuehui; Yang, Zhangsheng; Tong, Xiaohang; Zhao, Qi; Xu, Yuanyuan; Deng, Hongyu; Bartlam, Mark; Rao, Zihe
Title: Crystal Structure of the C-Terminal Cytoplasmic Domain of Non-Structural Protein 4 from Mouse Hepatitis Virus A59 Document date: 2009_7_10
ID: 1beonuh7_32
Snippet: Crystallization MHV-A59 nsp4C was crystallized by the hanging drop vapor diffusion method with a 2 mL drop volume at 18uC. 1.0 mL nsp4C at 3 mg ml 21 was added to the same volume of reservoir solution (10% PEG MME550, 0.1 M glycine, pH 9.0, and 0.01 M zinc sulfate), producing plate like crystals belonging to the space group of C2. Crystals of the selenomethionyl (Se-Met) substituted nsp4C were obtained from the same conditions to solve the phase .....
Document: Crystallization MHV-A59 nsp4C was crystallized by the hanging drop vapor diffusion method with a 2 mL drop volume at 18uC. 1.0 mL nsp4C at 3 mg ml 21 was added to the same volume of reservoir solution (10% PEG MME550, 0.1 M glycine, pH 9.0, and 0.01 M zinc sulfate), producing plate like crystals belonging to the space group of C2. Crystals of the selenomethionyl (Se-Met) substituted nsp4C were obtained from the same conditions to solve the phase problem. The Se-Met crystals shared the same space group and similar unit cell dimensions as the wild-type (WT) crystals. The WT and Se-Met derivative crystals of nsp4C were highly susceptible to nucleation and twinning. Crystals of the C425S mutant belonging to the space group P4 1 2 1 2 were obtained under the condition of 26% PEG MME550, 0.1 M glycine pH 9.0, and 0.01 M zinc sulfate with a protein concentration at 6 mg ml 21 .
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