The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p

Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H

In our previous study, LPS triggered CD68-expressing macrophage infiltration and OFSCs inhibited activation of the toll-like receptor 4/CD14/iNOS pathway in lung parenchyma [23] . In the present study, macrophages were treated with LPS at various concentrations (10 to 1,000 ng/ml) for 6 hours. LPS dose-dependently increased the expression of CD68, iNOS, and TNFα in macrophages ( Figure 1A , left panel), and 100 ng/ml LPS and above significantly triggered the CD68 expression and iNOS production in macrophages ( Figure 1A , right panel). A LPS concentration of 100 ng/ml was thus chosen for further experiments.