Selected article for: "confocal microscopy and fluorescence confocal microscopy"
Author: Wilton T. Snead; Wade F. Zeno; Grace Kago; Ryan W. Perkins; J Blair Richter; Chi Zhao; Eileen M. Lafer; Jeanne C. Stachowiak
Title: BAR scaffolds drive membrane fission by crowding disordered domains
  • Document date: 2018_3_4
    • ID: drqseaaa_103
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/276147 doi: bioRxiv preprint PtdIns(4,5)P 2 , 2 mol% DP-EG10-biotin, and 2 mol% Oregon Green 488-DHPE. For vesicles in (F), DPPC and DPPS were replaced with DOPC and DOPS, respectively. All vesicles extruded to 200 nm. (A) Two representative electron micrographs of tubules generated by 33 µM F-BAR. Dashed boxes indicate zoomed reg.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/276147 doi: bioRxiv preprint PtdIns(4,5)P 2 , 2 mol% DP-EG10-biotin, and 2 mol% Oregon Green 488-DHPE. For vesicles in (F), DPPC and DPPS were replaced with DOPC and DOPS, respectively. All vesicles extruded to 200 nm. (A) Two representative electron micrographs of tubules generated by 33 µM F-BAR. Dashed boxes indicate zoomed regions to the right of each image. Black arrows indicate tubules. Scale bars, including zoomed regions: 200 nm. (B) Histogram of the outer diameters of tubules generated by 33 µM F-BAR. Mean = 21 ± 2 nm first s.d., n = 524 tubules. Movie S4. Full-length amphiphysin drives collapsing of vesicles into diffraction-limited tubules and fragments. GUV composition: 79.5 mol% DOPC, 5 mol% PtdIns(4,5)P 2 , 15 mol% DOPS, 0.5 mol% Oregon Green 488-DHPE. GUVs were mixed with 5 µM Amph-FL and imaged by confocal microscopy. Fluorescence signal comes from Atto594-labeled protein. The frames are approximately 400 ms apart. The video plays at 5 frames per second.

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