Author: Zhang, Chi; Xiu, Leshan; Xiao, Yan; Xie, Zhengde; Ren, Lili; Peng, Junping
Title: Simultaneous Detection of Key Bacterial Pathogens Related to Pneumonia and Meningitis Using Multiplex PCR Coupled With Mass Spectrometry Document date: 2018_4_5
ID: 0k2blp7n_21
Snippet: In this study, we developed a 12-plex method to simultaneously detect 11 key bacterial pathogens associated with pneumonia and meningitis, using HBB as a nucleic acid extraction control. On the basis of previously published studies, we chose well-characterized and highly sensitive and specific genes as targets. In silico analysis showed that the BP-MS method can be used to specifically detect target pathogens, including most strains within a spec.....
Document: In this study, we developed a 12-plex method to simultaneously detect 11 key bacterial pathogens associated with pneumonia and meningitis, using HBB as a nucleic acid extraction control. On the basis of previously published studies, we chose well-characterized and highly sensitive and specific genes as targets. In silico analysis showed that the BP-MS method can be used to specifically detect target pathogens, including most strains within a species. The final target genes and assays are listed in Table S1 in the Supplementary Material.
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