Selected article for: "lc system and tag database"
Author: Tchitchek, Nicolas; Eisfeld, Amie J; Tisoncik-Go, Jennifer; Josset, Laurence; Gralinski, Lisa E; Bécavin, Christophe; Tilton, Susan C; Webb-Robertson, Bobbie-Jo; Ferris, Martin T; Totura, Allison L; Li, Chengjun; Neumann, Gabriele; Metz, Thomas O; Smith, Richard D; Waters, Katrina M; Baric, Ralph; Kawaoka, Yoshihiro; Katze, Michael G
Title: Specific mutations in H5N1 mainly impact the magnitude and velocity of the host response in mice
  • Document date: 2013_7_29
    • ID: 1qc72ovc_65
    Snippet: Following lung and virus peptide/protein AMT tag database generation, capillary LC-MS analyses were performed on all individual H5N1 and mock-infected samples to generate quantitative data. For this, dried peptide samples were reconstituted in 30 μL of 25 mM ammonium bicarbonate, pH 7.8, and analyzed in duplicate and random order using identical chromatographic and electrospray conditions as for capillary LC-MS/MS analyses. The LC system was int.....
    Document: Following lung and virus peptide/protein AMT tag database generation, capillary LC-MS analyses were performed on all individual H5N1 and mock-infected samples to generate quantitative data. For this, dried peptide samples were reconstituted in 30 μL of 25 mM ammonium bicarbonate, pH 7.8, and analyzed in duplicate and random order using identical chromatographic and electrospray conditions as for capillary LC-MS/MS analyses. The LC system was interfaced to an ExactiveOrbitrap mass spectrometer (Thermo Scientific), and the temperature of the heated capillary and the ESI voltage were 250°C and 2.2 kV, respectively. Data were collected over a range of 400-2,000 m/z.

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