Selected article for: "input sample and low input sample"
Author: Tchitchek, Nicolas; Eisfeld, Amie J; Tisoncik-Go, Jennifer; Josset, Laurence; Gralinski, Lisa E; Bécavin, Christophe; Tilton, Susan C; Webb-Robertson, Bobbie-Jo; Ferris, Martin T; Totura, Allison L; Li, Chengjun; Neumann, Gabriele; Metz, Thomas O; Smith, Richard D; Waters, Katrina M; Baric, Ralph; Kawaoka, Yoshihiro; Katze, Michael G
Title: Specific mutations in H5N1 mainly impact the magnitude and velocity of the host response in mice
  • Document date: 2013_7_29
    • ID: 1qc72ovc_56
    Snippet: Lung tissues were directly submerged in RNA-Later stabilization solution (Life Technologies) following dissection, placed at 4°C overnight, followed by freezing at −80°C. Lung tissues were thawed, transferred to 1 ml TRIzol (Life Technologies) and homogenized using a TissueLyser or MagnaLyser. RNA was isolated using QiagenRNeasy Mini columns and the manufacturer's recommended protocol (Qiagen Inc., Valencia, CA). Fluorescently labeled probes .....
    Document: Lung tissues were directly submerged in RNA-Later stabilization solution (Life Technologies) following dissection, placed at 4°C overnight, followed by freezing at −80°C. Lung tissues were thawed, transferred to 1 ml TRIzol (Life Technologies) and homogenized using a TissueLyser or MagnaLyser. RNA was isolated using QiagenRNeasy Mini columns and the manufacturer's recommended protocol (Qiagen Inc., Valencia, CA). Fluorescently labeled probes were generated from each RNA sample using Agilent one-color Low Input Quick Amp Labeling Kit (Agilent Technologies). Individual cRNA samples were hybridized to oligonucleotide microarrays for gene expression profiling using Whole Mouse Genome Microarray Kit (Agilent Technologies). All the microarray experiments passed the quality control criteria of the Agilent Feature Extraction Software.

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