Author: Vojo Deretic; Graham S Timmins
Title: Azithromycin and ciprofloxacin have a chloroquine-like effect on respiratory epithelial cells Document date: 2020_3_31
ID: i4ijuk36_10
Snippet: We next tested AZT effects on P. aeruginosa adhesion to CF cells. We used AZT at a 200-fold lower concentration (1 µM) than its MIC (170 µM) against P. aeruginosa 40 . In addition, the monolayers were washed to eliminate extracellular AZT during bacterial adhesion assay. Pretreatment of IB3-1 (CF) cells with 1 µM AZT led to a decrease in P. aeruginosa binding by 85 ± 3% (Fig. 3B ). This result was confirmed using pCEP-R cells, the human norma.....
Document: We next tested AZT effects on P. aeruginosa adhesion to CF cells. We used AZT at a 200-fold lower concentration (1 µM) than its MIC (170 µM) against P. aeruginosa 40 . In addition, the monolayers were washed to eliminate extracellular AZT during bacterial adhesion assay. Pretreatment of IB3-1 (CF) cells with 1 µM AZT led to a decrease in P. aeruginosa binding by 85 ± 3% (Fig. 3B ). This result was confirmed using pCEP-R cells, the human normal bronchial epithelial cells 16HBE transfected with R-region of CFTR. Pretreatment of pCEP-R (CF) cells with 1 µM AZT led to a decrease of P. aeruginosa binding by 75 ± 7% (n = 6, P = 0.0002) (Fig. 3C) , confirming the findings with IB3-1 CF cells.
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