Author: Kouokam, Joseph Calvin; Lasnik, Amanda B.; Palmer, Kenneth E.
Title: Studies in a Murine Model Confirm the Safety of Griffithsin and Advocate Its Further Development as a Microbicide Targeting HIV-1 and Other Enveloped Viruses Document date: 2016_11_17
ID: 096348l5_15
Snippet: Spleen cells were isolated according to a well-established procedure [19] using ammoniumchloride-potassium (ACK) lysis buffer to remove red blood cells. Spleen T-cell activation was studied by flow cytometry as described above with mouse PBMCs for the detection of CD4, CD25, and CD69. B-cell activation was studied in a similar fashion using a dual fluorescent staining with anti-mouse CD19 (FITC-conjugated) and B220 (PE-conjugated) antibodies purc.....
Document: Spleen cells were isolated according to a well-established procedure [19] using ammoniumchloride-potassium (ACK) lysis buffer to remove red blood cells. Spleen T-cell activation was studied by flow cytometry as described above with mouse PBMCs for the detection of CD4, CD25, and CD69. B-cell activation was studied in a similar fashion using a dual fluorescent staining with anti-mouse CD19 (FITC-conjugated) and B220 (PE-conjugated) antibodies purchased from BD Pharmingen (San Jose, CA, USA).
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