Author: Fan, Qing; Kopp, Sarah J.; Connolly, Sarah A.; Longnecker, Richard
Title: Structure-Based Mutations in the Herpes Simplex Virus 1 Glycoprotein B Ectodomain Arm Impart a Slow-Entry Phenotype Document date: 2017_5_16
ID: 1v6nf28a_36
Snippet: Virus binding assay. HSV-1 gB 3A or GS3217 (WT) virus was diluted to 200 PFU/ml in PBS with 1% heat-inactivated calf serum and 1% glucose, and 1 ml was added to Vero cell monolayers in six-well plates at 4°C or 37°C. Plates were incubated for 0, 20, 40, 60, 80, 100, or 120 min. The cells were rinsed twice with PBS, overlaid with methylcellulose (Sigma-Aldrich) medium, and incubated at 37°C for 3 days. Plaques were detected by Giemsa staining......
Document: Virus binding assay. HSV-1 gB 3A or GS3217 (WT) virus was diluted to 200 PFU/ml in PBS with 1% heat-inactivated calf serum and 1% glucose, and 1 ml was added to Vero cell monolayers in six-well plates at 4°C or 37°C. Plates were incubated for 0, 20, 40, 60, 80, 100, or 120 min. The cells were rinsed twice with PBS, overlaid with methylcellulose (Sigma-Aldrich) medium, and incubated at 37°C for 3 days. Plaques were detected by Giemsa staining.
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