Structure-Based Design of Head-Only Fusion Glycoprotein Immunogens for Respiratory Syncytial Virus

Boyington, Jeffrey C.; Joyce, M. Gordon; Sastry, Mallika; Stewart-Jones, Guillaume B. E.; Chen, Man; Kong, Wing-Pui; Ngwuta, Joan O.; Thomas, Paul V.; Tsybovsky, Yaroslav; Yang, Yongping; Zhang, Baoshan; Chen, Lei; Druz, Aliaksandr; Georgiev, Ivelin S.; Ko, Kiyoon; Zhou, Tongqing; Mascola, John R.; Graham, Barney S.; Kwong, Peter D.

By contrast, mice primed with DS-Cav1 and boosted with i-693 or i-447, elicited RSV-neutralizing responses that were statistically comparable to those boosted with DS-Cav1. Importantly, focusing of overall antibody response to both antigenic sites Ø and II was enhanced in i-693 and i-447-boosted mice relative to a DS-Cav1 boost (Fig 5A) . Despite the smaller size of i-693 relative to i-447, the dimeric nature and flexibility of i-693 may have facilitated divalent antibody binding and thus enhanced boosting by i-693.