Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site Document date: 2015_3_25
ID: 10p3mth2_20
Snippet: We first addressed in the current study whether the role of the GGG intercodon was solely structural in supporting frameshifting in the complete frameshift element [35] by analysing its role using a dual luciferase reporter system in cultured COS-7 cells (Fig. 2) . With the natural GGG as the intercodon, the frameshift efficiency was 9.4%, consistent with the 5-10% range found for viral production in vivo [13] , [49] . This suggested that, while .....
Document: We first addressed in the current study whether the role of the GGG intercodon was solely structural in supporting frameshifting in the complete frameshift element [35] by analysing its role using a dual luciferase reporter system in cultured COS-7 cells (Fig. 2) . With the natural GGG as the intercodon, the frameshift efficiency was 9.4%, consistent with the 5-10% range found for viral production in vivo [13] , [49] . This suggested that, while there may be global structural effects on the −1 PRF efficiency involving other distant parts of the RNA, the element itself is the major determinant [28] , [53] .
Search related documents:
Co phrase search for related documents- cultured cell and luciferase reporter: 1
- cultured cell and viral production: 1, 2
- current study and luciferase reporter: 1, 2, 3, 4, 5, 6, 7, 8, 9
- current study and major determinant: 1
- current study and structural effect: 1, 2, 3
- current study and viral production: 1, 2, 3, 4, 5, 6, 7, 8, 9
- frameshift efficiency and GGG intercodon: 1, 2, 3, 4, 5, 6, 7, 8
- frameshift efficiency and intercodon natural ggg: 1
- frameshift efficiency and luciferase reporter: 1, 2, 3, 4, 5, 6, 7
- frameshift efficiency and natural ggg: 1, 2
- frameshift efficiency and PRF efficiency: 1, 2, 3, 4, 5, 6, 7
- frameshift element and GGG intercodon: 1, 2, 3, 4, 5, 6, 7
- frameshift element and intercodon natural ggg: 1, 2
- frameshift element and luciferase reporter: 1, 2, 3, 4
- frameshift element and natural ggg: 1, 2
- frameshift element and PRF efficiency: 1, 2, 3, 4, 5
- GGG intercodon and intercodon natural ggg: 1, 2, 3
- GGG intercodon and luciferase reporter: 1, 2
- GGG intercodon and natural ggg: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date