Author: Mathew, Suneeth F.; Crowe-McAuliffe, Caillan; Graves, Ryan; Cardno, Tony S.; McKinney, Cushla; Poole, Elizabeth S.; Tate, Warren P.
Title: The Highly Conserved Codon following the Slippery Sequence Supports -1 Frameshift Efficiency at the HIV-1 Frameshift Site Document date: 2015_3_25
ID: 10p3mth2_31
Snippet: In contrast to a cell depleted of eRF1 with shRNA (see Fig. 4E ), increasing the concentration of eRF1 had no effect on frameshift efficiency in the naturally occurring HIV-1 −1 PRF element that contains a non-cognate GGG intercodon (Fig. 5E, left) . If the intercodon of the HIV-1 element was either of the cognate UGA or UAG stop codons, however, co-transfection with pcDNA-eRF1 resulted in a reduction of frameshift efficiency by 37% (P < 0.01) .....
Document: In contrast to a cell depleted of eRF1 with shRNA (see Fig. 4E ), increasing the concentration of eRF1 had no effect on frameshift efficiency in the naturally occurring HIV-1 −1 PRF element that contains a non-cognate GGG intercodon (Fig. 5E, left) . If the intercodon of the HIV-1 element was either of the cognate UGA or UAG stop codons, however, co-transfection with pcDNA-eRF1 resulted in a reduction of frameshift efficiency by 37% (P < 0.01) and 20% (P < 0.05), respectively (Fig. 5E, right) . These data imply that the intercodon can be decoded prior to frameshifting, and is available for functional decoding by eRF1 when the GGG intercodon is replaced by a stop codon.
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