Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Document date: 2017_1_12
ID: 0238bsxb_5
Snippet: Vero cells and DF1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (final concentration 100 U/mL), and streptomycin (final concentration 100 μg/mL). A549 cells were cultured in F-12K medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS). All cells were purchased from American Type Culture Collection and incubated .....
Document: Vero cells and DF1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (final concentration 100 U/mL), and streptomycin (final concentration 100 μg/mL). A549 cells were cultured in F-12K medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS). All cells were purchased from American Type Culture Collection and incubated at 37˚C in a 5% CO 2 environment. IBV Beaudette strain [32] was passaged in Vero cells. Viral stocks were stored at -80˚C prior to use. The 50% tissue culture infective doses (TCID 50 ) of viral stocks were calculated by Reed-Muench method.
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