Author: Guo, Huichen; Huang, Mei; Yuan, Quan; Wei, Yanquan; Gao, Yuan; Mao, Lejiao; Gu, Lingjun; Tan, Yong Wah; Zhong, Yanxin; Liu, Dingxiang; Sun, Shiqi
Title: The Important Role of Lipid Raft-Mediated Attachment in the Infection of Cultured Cells by Coronavirus Infectious Bronchitis Virus Beaudette Strain Document date: 2017_1_12
ID: 0238bsxb_5
Snippet: Vero cells and DF1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (final concentration 100 U/mL), and streptomycin (final concentration 100 μg/mL). A549 cells were cultured in F-12K medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS). All cells were purchased from American Type Culture Collection and incubated .....
Document: Vero cells and DF1 cells were cultured in Dulbecco's modified Eagle's medium (DMEM, Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS), penicillin (final concentration 100 U/mL), and streptomycin (final concentration 100 μg/mL). A549 cells were cultured in F-12K medium (Life Technologies) supplemented with 10% fetal bovine serum (FBS). All cells were purchased from American Type Culture Collection and incubated at 37˚C in a 5% CO 2 environment. IBV Beaudette strain [32] was passaged in Vero cells. Viral stocks were stored at -80˚C prior to use. The 50% tissue culture infective doses (TCID 50 ) of viral stocks were calculated by Reed-Muench method.
Search related documents:
Co phrase search for related documents- bovine serum and fetal bovine serum: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82, 83, 84
- bovine serum and final concentration: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
- bovine serum and μg ml: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48
- bovine serum and infective dose: 1, 2, 3
- bovine serum and tissue culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32
- bovine serum and tissue culture infective dose: 1, 2, 3
- bovine serum and Vero cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- bovine serum and viral stock: 1, 2, 3
- DF1 cell and infective dose: 1
- DF1 cell and tissue culture: 1
- DF1 cell and tissue culture infective dose: 1
- DF1 cell and Vero cell: 1
- FBS fetal bovine serum and fetal bovine serum: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81, 82
- FBS fetal bovine serum and final concentration: 1, 2, 3, 4, 5
- FBS fetal bovine serum and μg ml: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- FBS fetal bovine serum and infective dose: 1
- FBS fetal bovine serum and tissue culture: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
- FBS fetal bovine serum and tissue culture infective dose: 1
- FBS fetal bovine serum and Vero cell: 1, 2, 3, 4, 5, 6, 7, 8
Co phrase search for related documents, hyperlinks ordered by date