Author: Tchitchek, Nicolas; Eisfeld, Amie J; Tisoncik-Go, Jennifer; Josset, Laurence; Gralinski, Lisa E; Bécavin, Christophe; Tilton, Susan C; Webb-Robertson, Bobbie-Jo; Ferris, Martin T; Totura, Allison L; Li, Chengjun; Neumann, Gabriele; Metz, Thomas O; Smith, Richard D; Waters, Katrina M; Baric, Ralph; Kawaoka, Yoshihiro; Katze, Michael G
Title: Specific mutations in H5N1 mainly impact the magnitude and velocity of the host response in mice Document date: 2013_7_29
ID: 1qc72ovc_52
Snippet: Lung tissues were homogenized using a TissueLyser (Qiagen) or MagnaLyser (Roche) in 1 ml of MEM containing a penicillin/streptomycin mixture (Life Technologies), and following centrifugation to remove debris, virus titrations were performed in Madin-Darby Canine Kidney (MDCK) cells using standard methods. Virus titers are represented as the total number of infectious viruses isolated per lobe......
Document: Lung tissues were homogenized using a TissueLyser (Qiagen) or MagnaLyser (Roche) in 1 ml of MEM containing a penicillin/streptomycin mixture (Life Technologies), and following centrifugation to remove debris, virus titrations were performed in Madin-Darby Canine Kidney (MDCK) cells using standard methods. Virus titers are represented as the total number of infectious viruses isolated per lobe.
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