Selected article for: "cytometry analysis and flow cytometry analysis"
Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p
  • Document date: 2014_8_13
    • ID: 1i6hni9s_10
    Snippet: In a transwell culture system, macrophages (5 × 10 5 /well for protein analysis and flow cytometry, and 1 × 10 5 /well for other experiments in this study) with or without LPS stimulation were seeded into 0.4 μm pore inserts of transwells (BD Falcon, Franklin Lakes, NJ, USA) and co-cultured with various numbers of OFSCs. The ratio of OFSC numbers versus macrophage numbers in the transwell system was adjustable from 0.5 to 4. During the period .....
    Document: In a transwell culture system, macrophages (5 × 10 5 /well for protein analysis and flow cytometry, and 1 × 10 5 /well for other experiments in this study) with or without LPS stimulation were seeded into 0.4 μm pore inserts of transwells (BD Falcon, Franklin Lakes, NJ, USA) and co-cultured with various numbers of OFSCs. The ratio of OFSC numbers versus macrophage numbers in the transwell system was adjustable from 0.5 to 4. During the period of coculture, cells were incubated in 1:1 mixed medium (one-half DMEM/Ham's F12 medium for macrophage and one-half IMDM containing 1% fetal bovine serum for OFSCs). After co-culture for 6 hours, a series of experiments were performed on macrophages and OFSCs, respectively.

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