Author: Wang, Qing S.; Jan, Eric
Title: Switch from Cap- to Factorless IRES-Dependent 0 and +1 Frame Translation during Cellular Stress and Dicistrovirus Infection Document date: 2014_8_4
ID: 0if5z3xp_36
Snippet: IGR IRES activity is stimulated during cellular stress and virus infection. We have also found that sequences adjacent to the IRES contribute to translational activity, which is similar to other findings [19, 25, 31, 33] . Moreover, inclusion of the downstream sequence of the IRES may negatively affect reporter enzymatic activity as we observed with fusion of ORFx with firefly luciferase. The construction of the T2A-containing bicistronic reporte.....
Document: IGR IRES activity is stimulated during cellular stress and virus infection. We have also found that sequences adjacent to the IRES contribute to translational activity, which is similar to other findings [19, 25, 31, 33] . Moreover, inclusion of the downstream sequence of the IRES may negatively affect reporter enzymatic activity as we observed with fusion of ORFx with firefly luciferase. The construction of the T2A-containing bicistronic reporter RNA along with the inclusion of adjacent sequences of the IRES should prove useful for expression of exogenous genes using the IGR IRES.
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