Technologies for Proteome-Wide Discovery of Extracellular Host-Pathogen Interactions

Martinez-Martin, Nadia

Monoclonal antibodies CD4 identified as the receptor for HIV infection of T cells [23, 24] Rhinovirus Monoclonal antibodies ICAM-1 as the common entry receptor for most rhinovirus serotypes [25, 26] Plasmodium falciparum AVEXIS BASIGIN identified as the cell-surface receptor that mediates erythrocyte infection [27] Human adenoviruses Extracellular human protein microarrays Elucidation of the extracellular interactome of adenovirus-encoded immunomodulatory proteins [28] Pseudomonas aeruginosa Extracellular pathogen protein microarrays (NAPPA) Screening of patient sera against all P. aeruginosa extracellular proteins. 12 proteins identified as potent antigens [29] Varicella zoster virus (VZV) Extracellular pathogen protein microarrays (NAPPA) Identification of 18 extracellular viral proteins that promote humoral responses upon screening of the entire VZV proteome [30] Streptococcus Extracellular pathogen protein microarrays Identification of new streptococcal proteins that interact with fibronectin, fibrinogen, and C4BP factors [31] Hepatitis delta virus LHDAg antigen Plasma membrane microarrays (MPA) 150 candidate interactions identified between viral antigen and plasma membrane proteins [32] Simian virus 40 (SV40) Plasma membrane microarrays (MPA) 99 candidate interactions between whole particles and plasma membrane proteins identified [32] Vaccinia virus (VACV) TRICEPS (MS) 7 candidate cell surface binding partners identified for VACV [33] Viral pathogens Computational studies Insights into global principles of virus-host PPI networks [15] [16] [17] [18] [34] [35] [36] Hepatitis C virus (HCV) cDNA libraries CD81, Claudin-1, and Occludin as cell surface receptors and some of the players involved in HSV internalization [37] [38] [39] Adenovirus and coxsackievirus B