Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p Document date: 2014_8_13
ID: 1i6hni9s_13
Snippet: For cell cycle analysis, cells were harvested with cell scraper, washed twice and fixed in 70% ethanol at -20°C. Nuclear DNA was stained with propidium iodide (50 mg/ ml). After blocking with Fc receptor for 15 minutes at 4°C, CD 206 or isotype-matched control antibody (BD Biosciences, San Jose, CA, USA) was added and incubated for 30 minutes at 4°C in the dark and was analyzed using FACSCalibur (Becton Dickinson, Franklin Lakes, NJ, USA) and .....
Document: For cell cycle analysis, cells were harvested with cell scraper, washed twice and fixed in 70% ethanol at -20°C. Nuclear DNA was stained with propidium iodide (50 mg/ ml). After blocking with Fc receptor for 15 minutes at 4°C, CD 206 or isotype-matched control antibody (BD Biosciences, San Jose, CA, USA) was added and incubated for 30 minutes at 4°C in the dark and was analyzed using FACSCalibur (Becton Dickinson, Franklin Lakes, NJ, USA) and FlowJo software (Tree Star, Ashland, OR, USA).
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