Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p Document date: 2014_8_13
ID: 1i6hni9s_25
Snippet: Male Balb/c mice were maintained in the animal facility and all experimental protocols were approved by the animal use and care committee of Taipei Medical University-Wan Fang Hospital. The animal model of LPS-induced ALI was established as per our previous report [23] . Briefly, LPS (25 μg in 50 μl sterile saline/mice) was delivered into 8-week-old to 10-week-old mice (BioLASCO Taiwan Co., Ltd, Taipei, Taiwan) via intratracheal injection. Twen.....
Document: Male Balb/c mice were maintained in the animal facility and all experimental protocols were approved by the animal use and care committee of Taipei Medical University-Wan Fang Hospital. The animal model of LPS-induced ALI was established as per our previous report [23] . Briefly, LPS (25 μg in 50 μl sterile saline/mice) was delivered into 8-week-old to 10-week-old mice (BioLASCO Taiwan Co., Ltd, Taipei, Taiwan) via intratracheal injection. Twenty minutes after LPS injection, 3 × 10 5 OFSCs with or without transfection of miR-671-5p inhibitor in 50 μl phosphate-buffered saline were administrated via the tail vein. Tail vein injection of 50 μl phosphate-buffered saline served as the control. Animals were sacrificed 6 hours after LPS exposure. Lung tissues were removed and stained with hematoxylin and eosin. Each condition was repeated in at least three independent mice.
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