Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p Document date: 2014_8_13
ID: 1i6hni9s_27
Snippet: The malondialdehyde level in lung tissue was detected by the Lipid Peroxidation (MDA) Assay Kit (Abcam). Tissue (10 mg) was homogenized on ice in 300 μl of the MDA Lysis Buffer (Abcam) and then centrifuged at 13,000 × g for 10 minutes to remove insoluble materials. Then 200 μl of the supernatant and 600 μl TBA solution were incubated at 95°C for 60 minutes before cooling down to room temperature in the ice bath for 10 minutes. The absorbance.....
Document: The malondialdehyde level in lung tissue was detected by the Lipid Peroxidation (MDA) Assay Kit (Abcam). Tissue (10 mg) was homogenized on ice in 300 μl of the MDA Lysis Buffer (Abcam) and then centrifuged at 13,000 × g for 10 minutes to remove insoluble materials. Then 200 μl of the supernatant and 600 μl TBA solution were incubated at 95°C for 60 minutes before cooling down to room temperature in the ice bath for 10 minutes. The absorbance at 532 nm was read and proportioned to the malondialdehyde level.
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