Author: Lu, Yujie; Liu, Wenjuan; Zhang, Man; Deng, Yanfang; Jiang, Min; Bai, Gang
Title: The Screening Research of NF-?B Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS Document date: 2019_3_3
ID: 1ahxau9k_12
Snippet: Activity. Dualluciferase assay system was used to verify the antiinflammatory activity of the monomeric compounds. The operation steps were the same as above. Additionally, Evidence-Based Complementary and Alternative Medicine 3 human IL-6 and IL-1 ELISA kits were used, respectively, to measure the concentrations of inflammatory factors (IL-6 and IL-1 ) in the culture supernatants of BEAS-2B cells after the stimulation of drugs and TNF-. The abso.....
Document: Activity. Dualluciferase assay system was used to verify the antiinflammatory activity of the monomeric compounds. The operation steps were the same as above. Additionally, Evidence-Based Complementary and Alternative Medicine 3 human IL-6 and IL-1 ELISA kits were used, respectively, to measure the concentrations of inflammatory factors (IL-6 and IL-1 ) in the culture supernatants of BEAS-2B cells after the stimulation of drugs and TNF-. The absorbance of each sample was measured at 450 nm using a Bio-Rad Model 680 microplate reader. Data processing followed the instructions of ELISA kits.
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