The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p

Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H

To determine the paracrine potency of OFSCs inhibiting iNOS production from LPS-activated macrophages, macrophages were treated for 6 hours with condition medium collected from various numbers of OFSCs depending on the ratio of OFSCs versus macrophages (OFSC/macrophage ratio from 0.5 to 4). OFSCs-CM dose-dependently decreased the iNOS production in macrophages induced by LPS ( Figure 1B , left panel). OFSC/macrophage ratio up to 1 and higher significantly inhibited LPS-triggered iNOS production in macrophages ( Figure 1B) . However, neither 100 ng/ml LPS ( Figure 1C , middle) nor OFSC-CM from OFSC/macrophage ratio of 1 ( Figure 1C , right) altered CD206, a well-known marker for the M2 phenotype [30] , on macrophages in the first 6 hours ( Figure 1C , left).