Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p Document date: 2014_8_13
ID: 1i6hni9s_34
Snippet: To study the paracrine effect of OFSCs on macrophage proliferation, numbers of macrophages were counted before and after treatment of OFSCs-CM with various concentrations for 6 hours. OFSC/macrophage ratio of 2 and higher significantly decreased macrophage numbers under LPS stimulation (Figure 2A) . Flow cytometry on cell cycle analysis demonstrated that both OFSCs-CM and noncontact culture with OFSCs increased the G0/ G1 population of macrophage.....
Document: To study the paracrine effect of OFSCs on macrophage proliferation, numbers of macrophages were counted before and after treatment of OFSCs-CM with various concentrations for 6 hours. OFSC/macrophage ratio of 2 and higher significantly decreased macrophage numbers under LPS stimulation (Figure 2A) . Flow cytometry on cell cycle analysis demonstrated that both OFSCs-CM and noncontact culture with OFSCs increased the G0/ G1 population of macrophages ( Figure 2B ). Quantitative analysis for regulators of G1/S transition by western blot revealed that G1/S promoting factors such as cyclin D 1 , CDK4, and CDK6 in macrophages were reduced, while two CDK inhibitors (p21 cip1 and p27 kip1 ) were activated by OFSCs-CM ( Figure 2C ).
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