Selected article for: "bovine serum and cell culture medium"
Author: Lien, Gi-Shih; Liu, Jen-Fang; Chien, Ming-Hsien; Hsu, Wei-Tse; Chang, Tzu-Hao; Ku, Chia-Chi; Ji, Andrea Tung-Qian; Tan, Peng; Hsieh, Ting-Lieh; Lee, Liang-Ming; Ho, Jennifer H
Title: The ability to suppress macrophage-mediated inflammation in orbital fat stem cells is controlled by miR-671-5p
  • Document date: 2014_8_13
    • ID: 1i6hni9s_9
    Snippet: Macrophage-orbital fat-derived stem cell co-culture studies Macrophages were incubated with serum-free medium (DMEM/Ham's F12 medium) for 24-hour starvation before co-culture with OFSCs or treated OFSCs-CM. OFSCs were maintained in low serum medium (Iscove's Modified Dulbecco's Media (IMDM) containing 1% fetal bovine serum) for 24-hour starvation before co-culture with macrophages or condition medium collection. In this study, Escherichia coli O5.....
    Document: Macrophage-orbital fat-derived stem cell co-culture studies Macrophages were incubated with serum-free medium (DMEM/Ham's F12 medium) for 24-hour starvation before co-culture with OFSCs or treated OFSCs-CM. OFSCs were maintained in low serum medium (Iscove's Modified Dulbecco's Media (IMDM) containing 1% fetal bovine serum) for 24-hour starvation before co-culture with macrophages or condition medium collection. In this study, Escherichia coli O55:B5-produced LPS (Sigma-Aldrich, St Louis, MO, USA) was added to the co-culture system or OFSC-CM-treated macrophages at a final concentration of 10 to 1,000 ng/ml.

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