The Screening Research of NF-?B Inhibitors from Moutan Cortex Based on Bioactivity-Integrated UPLC-Q/TOF-MS

Lu, Yujie; Liu, Wenjuan; Zhang, Man; Deng, Yanfang; Jiang, Min; Bai, Gang

A Waters Acquity UPLC System (Waters Co., Milford, MA, USA) equipped with a photo diode array detector (PDAD) was used to analyze samples. The chromatographic column was an Acquity BEH C 18 -column (2.1 mm ×100 mm, 1.7 m; Waters Co.). The flow rate was 0.40 mL/min and the column temperature was maintained at 30 ∘ C. The test sample injection volume was 5 L. The optimal mobile phase was composed of A (0.1% formic acid in water) and B (acetonitrile) and the ratio was as follows: 0-1.0 min, 2% to 5% B; 1.0-2.0 min, 5% to 6% B; 2.0-4.0 min, 6% to 7% B; 4.0-6.0 min, 7% to 9% B; 6.0-9.0 min, 9% to 14% B; 9.0-12.0 min, 14% to 16% B; 12.0-18.0 min, 16% to 21% B; 18.0-21.0 min, 21% to 27% B; 21.0-22.0 min, 27% to 40% B.