Selected article for: "gene expression and mass spectrometry"

Author: Tchitchek, Nicolas; Eisfeld, Amie J; Tisoncik-Go, Jennifer; Josset, Laurence; Gralinski, Lisa E; Bécavin, Christophe; Tilton, Susan C; Webb-Robertson, Bobbie-Jo; Ferris, Martin T; Totura, Allison L; Li, Chengjun; Neumann, Gabriele; Metz, Thomas O; Smith, Richard D; Waters, Katrina M; Baric, Ralph; Kawaoka, Yoshihiro; Katze, Michael G
Title: Specific mutations in H5N1 mainly impact the magnitude and velocity of the host response in mice
  • Document date: 2013_7_29
  • ID: 1qc72ovc_9
    Snippet: We collected 230 transcriptomic and 198 proteomic profiles from the lungs of 20-week old C57BL/6 mice infected with CA04-WT, VN1203-WT, VN1203-HAavir, VN1203-PB2627E, VN1203-NS1trunc, or VN1203-PB1F2del. Mice were infected with a range of inoculation dosages (from 10 2 to 10 6 Plaque Forming Unit -PFU) and lungs were harvested at 1, 2, 4 and 7 days post-infection (dpi) to measure gene expression using oligonucleotide arrays and obtain proteomic p.....
    Document: We collected 230 transcriptomic and 198 proteomic profiles from the lungs of 20-week old C57BL/6 mice infected with CA04-WT, VN1203-WT, VN1203-HAavir, VN1203-PB2627E, VN1203-NS1trunc, or VN1203-PB1F2del. Mice were infected with a range of inoculation dosages (from 10 2 to 10 6 Plaque Forming Unit -PFU) and lungs were harvested at 1, 2, 4 and 7 days post-infection (dpi) to measure gene expression using oligonucleotide arrays and obtain proteomic profiles by liquid chromatography-mass spectrometry (LC-MS). Time-matched lung samples from mock-infected mice were collected and a total number of 300 transcriptomic profiles and 266 proteomic profiles are represented in our dataset.

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