Author: Laine, Romain F.; Albecka, Anna; van de Linde, Sebastian; Rees, Eric J.; Crump, Colin M.; Kaminski, Clemens F.
Title: Structural analysis of herpes simplex virus by optical super-resolution imaging Document date: 2015_1_22
ID: 0zchxz00_45
Snippet: The coordinates of the centre of each particle identified by circular Hough transform were used for particle alignment. The aligned localizations were used to produce the high-density super-resolved image, using the same image reconstruction as that used by rapidSTORM. The diameter and thickness of each protein layer was obtained by weighted non-linear least-square fitting (Levenberg-Marquardt algorithm) of the radial distribution from the aligne.....
Document: The coordinates of the centre of each particle identified by circular Hough transform were used for particle alignment. The aligned localizations were used to produce the high-density super-resolved image, using the same image reconstruction as that used by rapidSTORM. The diameter and thickness of each protein layer was obtained by weighted non-linear least-square fitting (Levenberg-Marquardt algorithm) of the radial distribution from the aligned localization coordinates to that of the MCV model. The goodness of fit was assessed by calculating the corresponding reduced w 2 parameter as shown in equation (1).
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