Selected article for: "1x protease inhibitor and protease inhibitor"
Author: Myllykoski, Matti; Kursula, Petri
Title: Structural aspects of nucleotide ligand binding by a bacterial 2H phosphoesterase
  • Document date: 2017_1_31
    • ID: 0a3okta0_39
    Snippet: LigT was overexpressed in the BL21(DE3) strain using ZYM-5052 autoinduction medium [35] , supplemented with 100 μg/ml ampicillin and 0.01% Antifoam 204 (Sigma). The expression culture was incubated at +37˚C for 24 h. Overexpression did not lead to any obvious toxic effects. The dry cell weight was around 12 g for 1 liter of culture. Cells were harvested by centrifugation and resuspended in lysis buffer containing 50 mM Na-HEPES (pH 7.5), 500 mM.....
    Document: LigT was overexpressed in the BL21(DE3) strain using ZYM-5052 autoinduction medium [35] , supplemented with 100 μg/ml ampicillin and 0.01% Antifoam 204 (Sigma). The expression culture was incubated at +37˚C for 24 h. Overexpression did not lead to any obvious toxic effects. The dry cell weight was around 12 g for 1 liter of culture. Cells were harvested by centrifugation and resuspended in lysis buffer containing 50 mM Na-HEPES (pH 7.5), 500 mM NaCl, 20 mM imidazole, 0.5 mM TCEP, and 1x EDTA-free protease inhibitor (Roche). The suspension was flash-frozen in liquid nitrogen and stored at -70˚C until use.

    Search related documents:
    Co phrase search for related documents
    • 1x Roche EDTA free protease inhibitor and EDTA free protease inhibitor: 1
    • 1x Roche EDTA free protease inhibitor and lysis buffer: 1
    • 1x Roche EDTA free protease inhibitor and Roche EDTA free protease inhibitor: 1
    • cell weight and dry cell weight: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • centrifugation harvest and liquid nitrogen: 1
    • EDTA free protease and lysis buffer: 1, 2, 3, 4, 5, 6
    • EDTA free protease and Roche EDTA free protease inhibitor: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • EDTA free protease inhibitor and lysis buffer: 1, 2, 3, 4, 5
    • EDTA free protease inhibitor and Roche EDTA free protease inhibitor: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • expression culture and liquid nitrogen: 1
    • expression culture and lysis buffer: 1
    • expression culture and mm tcep: 1
    • liquid nitrogen and lysis buffer: 1, 2, 3, 4, 5, 6, 7
    • liquid nitrogen and mm tcep: 1, 2, 3
    • lysis buffer and mm tcep: 1, 2, 3, 4, 5, 6
    • lysis buffer and Roche EDTA free protease inhibitor: 1, 2, 3, 4, 5