Author: Andrabi, Raiees; Pallesen, Jesper; Allen, Joel D.; Song, Ge; Zhang, Jinsong; de Val, Natalia; Gegg, Gavin; Porter, Katelyn; Su, Ching-Yao; Pauthner, Matthias; Newman, Amanda; Bouton-Verville, Hilary; Garces, Fernando; Wilson, Ian A.; Crispin, Max; Hahn, Beatrice H.; Haynes, Barton F.; Verkoczy, Laurent; Ward, Andrew B.; Burton, Dennis R.
Title: The Chimpanzee SIV Envelope Trimer: Structure and Deployment as an HIV Vaccine Template Document date: 2019_5_21
ID: 1ni7949q_56
Snippet: Statistical analysis was performed using Graph Pad Prism 7 for Mac, Graph Pad Software, San Diego, California, USA. The IC 50 virus neutralization and the EC 50 trimer binding Ab titers with MT145K were compared by two-tailed nonparametric Spearman correlation test with 95% Confidence interval. P value of less than 0.05 were treated as significant. Cryo-EM data were analyzed by a differenceof-Gaussian approach (DoG-Picker) to identify molecular p.....
Document: Statistical analysis was performed using Graph Pad Prism 7 for Mac, Graph Pad Software, San Diego, California, USA. The IC 50 virus neutralization and the EC 50 trimer binding Ab titers with MT145K were compared by two-tailed nonparametric Spearman correlation test with 95% Confidence interval. P value of less than 0.05 were treated as significant. Cryo-EM data were analyzed by a differenceof-Gaussian approach (DoG-Picker) to identify molecular projection images for further processing. Maximum Likelihood optimization (RELION) was utilized to determine a subset of data (44,301 molecular projection images) most suitable for refinement to highest possible resolution and to determine projection image Euler angles and planar shifts.
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