Author: Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo-Yun; Li, Kui
Title: The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function Document date: 2019_6_28
ID: 1nr0hggt_14
Snippet: Infections of primary mouse neuronal cultures were carried out at day 8 post-differentiation when medium was removed and the cells were washed twice with HBSS. Based on cell counts from 3 wells, neurons were either mock -infected (controls) or infected with ZIKV-PR-VABC59 at a MOI of 1 in NbActiv4 media for 2 h at 37ËšC; duplicate wells for each time point were used. Inoculum was then removed, cells washed twice with NbActiv4, and then incubated .....
Document: Infections of primary mouse neuronal cultures were carried out at day 8 post-differentiation when medium was removed and the cells were washed twice with HBSS. Based on cell counts from 3 wells, neurons were either mock -infected (controls) or infected with ZIKV-PR-VABC59 at a MOI of 1 in NbActiv4 media for 2 h at 37ËšC; duplicate wells for each time point were used. Inoculum was then removed, cells washed twice with NbActiv4, and then incubated in 0.5 ml of NbActiv4 media at 37ËšC, 5% CO 2 , 95% humidity. At 24 and 48 h after infection, 0.5 ml TRIzol was added to each well for RNA extraction and qPCR analysis.
Search related documents:
Co phrase search for related documents- cell count and time point: 1, 2, 3, 4, 5, 6, 7, 8
- cell twice wash and twice wash: 1
- primary mouse and qpcr analysis: 1
- primary mouse and RNA extraction: 1
- primary mouse and time point: 1
- qpcr analysis and RNA extraction: 1, 2, 3, 4, 5, 6, 7, 8, 9
- qpcr analysis and time point: 1
- RNA extraction and time point: 1, 2, 3, 4, 5, 6, 7
Co phrase search for related documents, hyperlinks ordered by date