Selected article for: "dt primer and Primer sequence"
Author: Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo-Yun; Li, Kui
Title: The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function
  • Document date: 2019_6_28
    • ID: 1nr0hggt_19
    Snippet: For miRNA detection, we synthesized oligonucleotide primers and adopted the protocol as described in the qSTAR miRNA qPCR detection system (Origene). In brief, after a poly(A) tailing procedure, a miR-oligo-dT primer with a sequence of 5'-GAACATGTCTGCGTATCTCAG ACTTCTGATTCACGCTTTTTTTTTTTTTTTTTTTVN-3', was used for reverse transcription of total small RNAs. Subsequently, SYBR green-based qPCR was performed to measure the levels of miRNAs of interes.....
    Document: For miRNA detection, we synthesized oligonucleotide primers and adopted the protocol as described in the qSTAR miRNA qPCR detection system (Origene). In brief, after a poly(A) tailing procedure, a miR-oligo-dT primer with a sequence of 5'-GAACATGTCTGCGTATCTCAG ACTTCTGATTCACGCTTTTTTTTTTTTTTTTTTTVN-3', was used for reverse transcription of total small RNAs. Subsequently, SYBR green-based qPCR was performed to measure the levels of miRNAs of interest relative to that of an internal control, i.e., U6 snRNA. The primers for qPCR detection included a miRNA/snRNA-specific forward primer and a universal reverse primer, as follows. miR-92a, 5'-TATTGCACTTGTCCCGGC-3' (forward); miR-21, 5'-TAGCT TATCAGACTGATGTTG-3' (forward); U6 snRNA, 5'-CTGCGCAAGGATGACACGC AA-3' (forward); and miR-Rev-universal, 5'-GAACATGTCTGCGTATCTC-3' (reverse).

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