Author: Yang, Darong; Li, Nan L.; Wei, Dahai; Liu, Baoming; Guo, Fang; Elbahesh, Husni; Zhang, Yunzhi; Zhou, Zhi; Chen, Guo-Yun; Li, Kui
Title: The E3 ligase TRIM56 is a host restriction factor of Zika virus and depends on its RNA-binding activity but not miRNA regulation, for antiviral function Document date: 2019_6_28
ID: 1nr0hggt_19
Snippet: For miRNA detection, we synthesized oligonucleotide primers and adopted the protocol as described in the qSTAR miRNA qPCR detection system (Origene). In brief, after a poly(A) tailing procedure, a miR-oligo-dT primer with a sequence of 5'-GAACATGTCTGCGTATCTCAG ACTTCTGATTCACGCTTTTTTTTTTTTTTTTTTTVN-3', was used for reverse transcription of total small RNAs. Subsequently, SYBR green-based qPCR was performed to measure the levels of miRNAs of interes.....
Document: For miRNA detection, we synthesized oligonucleotide primers and adopted the protocol as described in the qSTAR miRNA qPCR detection system (Origene). In brief, after a poly(A) tailing procedure, a miR-oligo-dT primer with a sequence of 5'-GAACATGTCTGCGTATCTCAG ACTTCTGATTCACGCTTTTTTTTTTTTTTTTTTTVN-3', was used for reverse transcription of total small RNAs. Subsequently, SYBR green-based qPCR was performed to measure the levels of miRNAs of interest relative to that of an internal control, i.e., U6 snRNA. The primers for qPCR detection included a miRNA/snRNA-specific forward primer and a universal reverse primer, as follows. miR-92a, 5'-TATTGCACTTGTCCCGGC-3' (forward); miR-21, 5'-TAGCT TATCAGACTGATGTTG-3' (forward); U6 snRNA, 5'-CTGCGCAAGGATGACACGC AA-3' (forward); and miR-Rev-universal, 5'-GAACATGTCTGCGTATCTC-3' (reverse).
Search related documents:
Co phrase search for related documents- forward primer and oligo dt: 1, 2, 3, 4
- forward primer and oligonucleotide primer: 1, 2, 3
- forward primer and qPCR detection: 1
- forward primer and reverse primer: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79, 80, 81
- forward primer and reverse transcription: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26
- forward primer and small rna: 1
- forward primer and universal reverse primer: 1
- internal control and oligonucleotide primer: 1
- internal control and qPCR detection: 1, 2, 3, 4, 5, 6, 7, 8, 9
- internal control and qPCR detection system: 1
- internal control and reverse primer: 1, 2
- internal control and reverse transcription: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43
- internal control and small rna: 1
- miRNA detection and small rna: 1
Co phrase search for related documents, hyperlinks ordered by date